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Abstracts of FIS/HIS 2016

Poster Presentations

Topic: Antimicrobials and antimicrobial resistance

ID: 4369

Antibiotic resistance patterns of bacterial isolates in adult

intensive care unit at Nizwa hospital, Oman

Nashwa Alkasaby.

Faculty of Medicine, Mansoura University, Egypt


Infection is a commonly encountered problem for

patients in intensive care units (ICUs) and Multidrug-resistant

(MDR) bacterial infection is predominant.


The aim of this study was to detect the frequency

of different bacterial isolates and their antibiotic susceptibility pattern

from patients admitted to adult ICU in a 5 year period from January

2008 to December 2012 at Nizwa hospital, Oman.


Different microbiological samples were collected and

analyzed by routine conventional methods at microbiology section,

laboratory department; Nizwa hospital. Antibiotic susceptibility (ABS)

test was done using modified Kirby-Bauer disk diffusion method as

per Clinical and Laboratory Standards Institute (CLSI) guidelines.


Total (3930) clinical samples were processed, out of which

12.8% (504/3930) showed evidence of infection, 73.6% (371/504) were

Gram-negative bacteria, 22.8% (115/504) were Gram-positive and 3.6%

(18/504) were


species. Respiratory tract infection was the

most common site of infection. Among the isolates, the most com-

monly found microorganism was

Pseudomonas aeruginosa

in respira-

tory samples, pus and wound infection, However


spp. and

Escherichia coli

were predominant in urinary tract infection. Coagulase



was the predominant in blood. Extended-



-lactamase (ESBL)-producing

Escherichia coli




and Proteus occurred in 43.2% (29/67), 28.6% (18/63) and 45.5% (5/11)

of total

Escherichia coli



spp. and Proteus isolates, respect-

ively. While 16.6% of

Staphylococcus aureus

isolates were Methicillin


Staphylococcus aureus


Discussion and/or Conclusion(s):

Adult ICUs are faced with the

increasingly rapid emergence and spread of antibiotic-resistant

bacteria. Excellent antibiotic policy and infection control implemen-

tation are important priorities for these critically ill patients.

ID: 4422

Extended-spectrum beta-lactamase producing Gram-negative

bacterial isolates causing nosocomial infection in Nepal: an

emerging threat to clinical therapeutics

Manoj Sah


, Ram Krishna


, Shyam Mishra


, Basiata Rijal



Bharat Mani Pokhrel




Kathmandu University, Kantipur Dental College

Teaching Hospital & Research Center,


Department of Microbiology,

Institute of Medicine, Tribhuvan University Teaching Hospital (TUTH),

Kathmandu, Nepal


Extended spectrum


-lactamase (ESBL)-producing

bacterial isolates causing nosocomial infection pose unique challenges

to medical practitioners now-a-days placing an extra burden on

individual patients and on healthcare system due to irrational uses of

antibiotics in Nepal.


The study was aimed to determine the ESBL-

producing bacterial isolates accountable for nosocomial infection.


Two hundred seventy nine nosocomial bacterial isolates

were studied over a period of one year from March 2011 to February

2012 at Department of Microbiology, Tribhuvan University Teaching

Hospital, Kathmandu, Nepal as described by American Society for

Microbiology (ASM). Antibiotic susceptibility testing was performed

by the Kirby-Bauer Disk Diffusionmethod as recommended by Clinical

and Laboratory Standards Institute (CLSI). A combination disk method

was done for detection of ESBL-production according to CLSI guide-

lines. Data were analyzed using SPSS 17.0.


Of the total isolates,

Escherichia coli

36.2% (n = 98),



species 27.7% (n = 75)

Klebsiella pneumoniae

18.5% (n = 50),

Pseudomonas aeruginosa

13.7% (n = 37) and

Citrobacter freundii


(n = 9). Alarmingly, 21.5% (n = 58) bacterial isolates were recovered

ESBL-producer in which

K. pneumoniae

was found to be predominant

26% (n = 13) followed by

E. coli

24.5% (n = 24),

P. aeruginosa


(n = 7), and


r species 17.3% (n = 13).

Discussion and/or Conclusion(s):

We found a high prevalence of

nosocomial bacteria producing ESBL in our hospital which prompts

a special attention for the management of such patients as well as

urgent need for implementation of infection control strategies to

prevent the dissemination of such strains. ESBL detection should be

routinely performed in clinical laboratory as false reporting would

results in treatment failure despite in vitro sensitivity.

ID: 4673

Analysis of the biofilm formation ability of antibiotic resistant

Escherichia coli


Klebsiella pneumoniae


Sinan Baho


, Shivanthi Samarasinghe






De Montfort University/

Faculty of Health and Life Sciences


Biofilm formation represents one of the major health

problems due to its difficulty to be eradicated. Most studies focus on

seeking for new antibiotics to treat biofilms but very little focus on the

bacterial virulence factors

genes related to biofilm formation.


In our work we are trying to find the relationship

between biofilm formation and Quorum Sensing (QS) virulence


genes. This might give new insights for using new therapeutic

alternatives other than the traditional antibiotic.


In this work, the amount of biofilm formation of the

antibiotic resistant strains of

Escherichia coli


Klebsiella pneumoniae

at different time points of growth (6, 12, 24 and 48 hours) was

determined using Biofilm Formation Assay (Tissue Culture Plate Assay;

TCP). Six wells and 96 wells tissue culture plates were used in this

study using LB broth and nutrient broth, and in static and shaking

incubation conditions.

Results: Results

showed that amount of biofilm produced varies

according to the bacterial growth stage at 6, 12, 24 and 48 hours of

growth and culture conditions (static or shaking) in addition to the

type of the growth media.

Discussion and/or Conclusion(s):

In conclusion, quantity of biofilm

produced is affected by the strain type, growth stage and culture

conditions. Using these result, next step will be the selection of

the highest biofilm producer strains for deletion of one of the QS

controlled virulence gene and study this effect on strain

s biofilm

formation ability.

ID: 4675

Comparing the feasibility and accuracy of wound aspiration and

wound swab in surgical site infection: a prospective study

Mohammad Razai.

London Northwest Healthcare


Surgical wound infections remain a significant clinical

problem. Currently wound swabs are preferred method to identify

causative organisms over wound aspiration biopsy.


This prospective study aimed to compare the

pathogenic bacteria isolated from wound aspirations and wound

swabs in patients who had empirical antimicrobial therapy. The

feasibility of the procedure and whether the patients experienced any

significant pain during or after aspirate sampling was also explored.


Seven patients with surgical site infection who had

antimicrobial therapy were included in a prospective comparative

study between wound aspiration and wound swabs.

0195-6701/© 2016 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.