The Journal of Hospital Infection - Supplement 1

Topic: Decontamination

ID: 4505

Advanced technology in combating multidrug-resistant HAIs

causing agents

Tatiana Grenkova

, Eugenia Selkova

, Margarita Gusarova

Marina Sukhina

G.N.Gabrichevskiy Moscow Research Institute of

Epidemiology and Microbiology, Russian Federation,

A.N.Ruzhih State

Scientific Centre for Coloproctology, Moscow, RF

Background:

A solution of HAI germs

’

increasing antibiotic and

disinfectant resistance problem requires development and implemen-

tation of new effective approaches to pathogens destruction.

Aim(s)/Objective(s):

To study an effectiveness of pulsed xenon

ultraviolet (UV) radiation continuous spectrum generated by

‘

Alfa-

’

irradiating device against clinical strains of MDR bacteria

’

s (

MRSA

VRE

P. aeruginosa

Acinetobacter baumannii

K. pneumonia

. difficile

spores and

Mycobacterium terrae

test strain.

Method(s):

Plastic and metal surfaces contaminated with the corres-

ponding bacteria and

. difficile

spores were exposed to radiation. The

initial contamination was 10

–

. The surfaces were placed vertically

and horizontally against the irradiator at 1.5

–

4-meters distance.

The exposure time was 5 and 10 minutes for bacteria, 4, 8, 16 and 30

minutes for

Mycobacteria

, and 4, 8, and 16 minutes for

C. difficile

spores. Sheep red blood cells were used as biological burden.

Results:

After 5-minute treatment of horizontally and vertically

aligned plastic and metal surfaces contaminated with bacterial

strains (except

. difficile

and mycobacteria) an effective decrease of

microbial count has reached from 4 lg to 7 lg. For mycobacteria and

C. difficile

spores on the surfaces placed vertically at 2-meter distance

from the irradiator, the 100% elimination of microorganisms was

achieved in 8 minutes, for 4-meter distance

–

in 16 minutes.

Discussion and/or Conclusion(s):

The high efficiency of the continu-

ous spectrum pulsed UV against all tested HAIs pathogens allows for

recommending the irradiating device with pulsed xenon lamps for

wide use at hospitals.

ID: 4553

Chlorhexidine-impregnated wipes to prevent multi-resistant

bacteria colonization in an intensive care unit

Jesus Ruiz

, Esther Villarreal

, Monica Gordon

, Juan Frasquet

Álvaro Castellanos

, Susana Cuesta

, Marcela Pieruccioni

Paula Ramirez

Hospital Universitario La Fe,

Intensive Care Unit.

Hospital Universitario La Fe

Aim(s)/Objective(s):

To assess the efficacy of chlorhexidine-impreg-

nated wipes [Clinell-Wash

(Vesismin/Ibersurgical)] to prevent multi-

resistant bacteria (MRB) colonization and infection in an intensive care

unit (ICU).

Method(s):

A prospective observational study in a 24-beds critical care

unit was designed. Chlorhexidine wipes were introduced for daily

washing of patients undergoing mechanical ventilation as for those

patients colonized by MRB.

To assess the efficacy of chlorhexidine wipes, evolution of number of

patients and ICU-stays withMRB colonizationwere analysed during 11

months. Number of nosocomial infections compared with the

equivalent period last year was analysed.

Data analysis was conducted through Stata software v.13.0, using Ji-

square test to compare proportions. Evolution of patient colonization

over the months was evaluated using a linear regression model.

Results:

During the intervention period, 1835 patients were admitted

to the ICU [mean age: 61.1 (SD: 15) years; APACHE II (Median (IQR): 30

(18

–

25)].

Following the introduction of chlorhexidine wipes, a significant

reduction in the incidence of patients with MRB colonization over

the months was observed (

−

0.04; r2 = 0.438; p = 0.027). No

significance reduction in number of ICU-stays with MRB colonization

was found (r2 = 0.03; p = 0.279). A significant reduction in the

overall incidence of nosocomial infection in the intervention period

compared to the equivalent period last year was observed (2.23% vs

3.91%; p = 0.004). No contact dermatitis was observed in treated

patients.

Discussion and/or Conclusion(s):

Chlorhexidine-impregnated wipes

reduces the incidence of colonization and infection by MRB, appearing

as a practical tool to prevent nosocomial infections in critical care

units.

ID: 4584

Value of clean monitoring in endoscope reprocessing

Henning Reuter

, Sabine Heiligtag

, Walter Popp

, Nina Parohl

Germany,

Hykomed GmbH

Background:

Recent reports of endoscope-associated outbreaks with

MDROs have shown that reprocessing of flexible endoscopes remains

a challenge. Improved quality control by close monitoring of the

cleaning of endoscopes can contribute to the identification of

endoscope at risk for patient to patient transmission.

Aim(s)/Objective(s):

Demonstrate contribution of clean monitoring

with Adenosine-triphosphate (ATP) bioluminescence test in the

reprocessing of flexible endoscopes to the overall process quality.

Method(s):

The bedside flush, manual cleaning and automated

endoscope reprocessing (AER) process steps of routine reprocessing

of 60 clinically used gastroscopes have been monitored on six non-

consecutive days with an ATP test in a German teaching hospital.

Results:

The study shows an unstable cleaning outcome of the

reprocessing. Although the cleaning performance of the manual

cleaning and the AER was relatively stable, the study suggests that the

endoscopic procedure or the effectiveness of the bedside flush drives

the overall outcome of the endoscope cleaning. Influence of the

condition of specific endoscopes and the invasiveness of the procedure

are shown.

Discussion and/or Conclusion(s):

Clean monitoring of the reproces-

sing of flexible endoscopes with a rapid, sensitive and quantitative ATP

test can help identifying endoscopes at risk to contribute to pathogen

transmission and can provide immediate feedback to the operator. A

systematic surveillance can provide improvement to the overall quality

control of reprocessing endoscopes and helps hospitals in document-

ing their process quality.

ID: 4686

Water, water, everywhere, and all the wards have sinks: a

demountable tapmixer assembly that can be processed in awasher

disinfector

Christina Bradley, Martyn Wilkinson, Adam Fraise.

Hospital Infection

Research Laboratory, Queen Elizabeth Hospital Birmingham

Background:

Infections with

Pseudomonas aeruginosa

have been

linked to hospital water supplies; the source in a Belfast outbreak

was thought to be handwash basin taps (ref). The guidance given in

HTM 04-01 Addendum states that healthcare premises should have

flushing regimes in place, and advise against discarding patient-

associated fluids, e.g. bathing water, down the sink. However, although

this may reduce or delay the formation of biofilm, it will not prevent it.

A tap capable of being easily removed from the plumbing system,

and being dismantled for thermal disinfection, has been developed

by Armitage Shanks. In this study the Markwik21 tap assembly

was artificially contaminated with a biofilm-producing strain of

P. aeruginosa

and processed in a validated benchtop thermal washer

disinfector (WD).

Method(s):

A Markwik21 tap was dismantled, each component

contaminated by immersion/flushing with a suspension of the test

organism, and left in a humid environment for 7 days. The components

were then processed in a WD. Sampling occurred before and after

disinfection to establish pre and post disinfection counts. Testing was

performed in triplicate.

Abstracts of FIS/HIS 2016

–

Poster Presentations / Journal of Hospital Infection 94S1 (2016) S24

–

S134

S64