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that dental handpieces used in invasive procedures should be steam
sterilized in a vacuum benchtop steam sterilizer.
ID: 5077
UMONIUM38 Neutralis securing of incubators in neonatalogy
Nicolas Coltel
1
, Frank van Laer
2
.
1
Huckert
’
s International,
2
UZA
Background:
Incubators could be source of contamination that could
affect neonates. Their disinfection procedure should be a key element
in the fight against pathogens, and should be a crucial point to control.
Aim(s)/Objective(s):
Assess the performance of a DM dedicated-
disinfectant during the maintenance of incubators. Identify potential
sources of infection. Evaluate sampling material for reliability and
accuracy.
Method(s):
15 incubators were sampled with Rodac plates and
environmental swabs before and after disinfetion. Rodac plates were
applied on mastress and swabs on doors. Tranpsort liquid form swabs
was spread on specific chromogenic media. Plates were identified and
blind-counted by different operators.
Results:
After disinfection, doors were free of contamination,
mattresses (2/15) presented a residual contamination (1 CFU) with
S. saprophyticus
or
Pseudomonas.
S. aureus
was more oftenly present on mattresses (9/15) than doors
(6/15) and was not observed anymore after disinfection.
When disinfection and sampling were performed by 2 different
operators, an incubator appeared really positive. Contamination after
disinfection? Inadequate disinfection?
RODAC plates are more reliable than 100 μL from a swab to detect a
potential contamination. Swab sampling followed by spreading on
chromogenic media allows the examination of the complexity of
bacterial flora.
Discussion and/or Conclusion(s):
Umonium38
®
Neutralis presented
bactericidal activity at 0.5% after 10 min. RODAC plates were a powerful
indicator of bacterial contamination.
Mattresses were more contaminated than doors, with bacteria from
enterobacteriae and/or Pseudomonas clusters. Mattress is a critical
item to be disinfected and monitored.
Disinfection controls could reveal possible lack of disinfecton or
treatment failure.
‘
Orientation
’
medium is sufficient to detect the
variety of bacteria.
ID: 5139
Department of Public Health Sciences and Pediatrics Torino
University
Carla Maria Zotti
1
, Savina Ditommaso
2
, Monica Giacomuzzi
2
,
Elisa Ricciardi
2
.
1
Department of Public Health Sciences and Pediatrics,
Torino University,
2
Department of Public Health Sciences and Pediatrics,
Torino University
Background:
DUWLs become contaminated from the main water
supply, which, although potable, still carries bacteria. Removal or
inactivation of bacteria requires the use of chemical germicides, which
could be used intermittently as a
‘
shock
’
treatment and/or continu-
ously in small quantities.
Aim(s)/Objective(s):
Longitudinal study was undertaken to find out
the efficacy of the continuous use of ICX
®
tablets, a chemical cleaner,
combined with monthly shock disinfection using hydrogen peroxide
(3%).
Method(s):
The DUWLs effluents were sampled to determine the
bacteria total viable counts (TVCs) from two dental chairs treated as
above and from one chair that never received treatments.
The efficacy of ICX
®
was also assessed in vitro versus the collection
strains (
Staphilococcus aureus
,
Pseudomonas aeruginosa
) according to
EN 1040 method.
Results:
The post-intervention monitoring achieved levels of TVCs
conforming to the limits of the Italian standard for drinking water.
However, during the six months of surveillance after 30 days of shock,
chairs showed an increase in TVCs. No significant differences were
found between the TVCs detected in the dental chairs receiving
treatments versus the control. In vitro ICX
®
caused 4.0 and 7.6 decimal
log reduction of
S. aureus
respectively at 60 min and after 24 h. Instead,
the product is less effective (1.3 log) in reducing the number of
P. aeruginosa
both after 60 min and 24 h.
Discussion and/or Conclusion(s):
In conclusion, a continuous dis-
infection with ICX
®
as proposed by the manufacturer, does not control
the bacterial growth during daily use; furthermore it has shown a poor
performance against gram-negative bacteria.
ID: 5160
Comparison of two UV systems for terminal disinfection of patient
rooms contaminated with MRSA, ESBL-producing
Klebsiella
pneumoniae
and
Clostridium difficile
Shanom Ali, Samuel Yui, Monika Muzslay, Peter Wilson.
University
College London Hospitals NHS Trust
Background:
Persistence of bacteria/spores in the clinical environ-
ment is a potential risk for acquisition of infection. UV-irradiation
(enhanced-disinfection) devices were evaluated for decontamination
efficacy.
Method(s):
Following terminal-disinfection of a single-isolation room,
test organims (
∼
10
5
cfu MRSA,
K. pneumoniae
[KP], 10
4
cfu
C. difficile
spores [CD]) prepared in low/heavy soil were inoculated onto 1 cm
2
carriers placed at various locations (1-Floor, 2-Under bed, 3-Footrail,
4-Headboard, 5-bedside table).
Devices:
A
–
Triple-emitter (254 nm) arranged around the bed in triangular
formation (Surfacide
®
Helios)
B
–
Single-emitter (265 nm) relocated according to sensors in room
(Ultra-V
™
, Hygiene Solutions)
Surface swabs were taken from 14 environmental sites before and after
terminal-disinfection and after enhanced-disinfection. Inoculated
carriers were assayed to determine efficacy (log-reductions).
Results:
Time for enhanced-disinfection was similar between devices
(A = 35 ± 14 min, B = 36 ± 6 min). All (14/14) environmental sites were
contaminated before terminal-disinfection and 13/14 sites (93%) after
(n = 27). Enhanced-disinfection eliminated contamination in 8/14
(57%) and 11/14 (79%) using device A or B respectively.
Both devices reduced MRSA and KP (>5 log reductions) regardless of
soiling or location and effective (>4 log reduction) against CD with low
soiling on: floor-surfaces, difficult-to-access areas (location 1
–
2) and
high-frequency-touch sites (location 3
–
4) but less effective (0.3 log
reduction) with heavy soiling. Device A effectively reduced >4 log CD
from the bedside table despite heavy soiling.
Conclusion:
Enhanced-disinfection (UV-irradiation) is effective
against MRSA, KP and CD spores and can reduce contamination from
surfaces missed during terminal cleaning. In-use efficacy-testing
should be considered to assure correct positioning of devices to
access shadowed areas, floor surfaces and improve hospital decon-
tamination protocols.
ID: 5179
In vitro efficacy of disinfectants utilised for skin decolonization
and environmental decontamination during a hospital outbreak
with
Candida auris
Silke Schelenz
1
, Alireza Abdolrasouli
2
, Darius Armstrong-James
2
.
1
Royal Brompton Hospital,
2
Imperial College School of Public Health
Background:
A recent
C. auris
outbreak has led to the implementation
of extensive Infection-Prevention-and-Control-measures.
C. auris
skin
and environmental decontamination has been a challenge as there is
no published information on the efficacy against
C. auris
.
Aim(s)/Objective(s):
To establish the
in-vitro
efficacy of
disinfectants
utilised for skin and environmental decontamination during a
C. auris
outbreak.
Method(s):
In-vitro
susceptibility of
C. auris
and ATCC controls
were tested against chlorhexidine, iodinated-povidone and sodium-
dichloroisocyanurate using a microdilution-method.
Abstracts of FIS/HIS 2016
–
Poster Presentations / Journal of Hospital Infection 94S1 (2016) S24
–
S134
S68