The Journal of Hospital Infection - Supplement 1 - November 2016

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detailing invasive pulmonary aspergillosis in previously immunocom-
petent patients with influenza A.
Aim(s)/Objective(s): We describe two suspected cases of invasive
pulmonary aspergillosis post influenza in previously immunocompe-
tent patients occurring in Glasgow in 2015 –2016.
Method(s): We discuss the details of these cases and compare to
previously published case reports of aspergillosis post influenza in the
literature.
Results: Invasive pulmonary aspergillosis can occur in previously
immunocompetent influenza A patients.
Discussion and/or Conclusion(s): Invasive pulmonary aspergillosis
is a rare complication during or following influenza infection and can
occur in previously immunocompetent patients with influenza A.
ID: 5140
A rare case of fungal keratitis
Tara Moshiri 1, Craig Wilde2, Susan Elizabeth Snape1. 1Department of
Microbiology, Nottingham University Hospitals, 2Department of
Ophthalmology, Nottingham University Hospitals
Background: Scopulariopsis species are common soil saprophytes
widely distributed in nature, previously isolated from plant debris, air
and moist indoor environments. S. brevicaulis appears to be the most
commonly isolated species with S. gracilis being the second most
common. Ocular involvement has only rarely been reported and there
remains a paucity of cases of Scopulariopsis keratitis within the
literature.
Aim(s)/Objective(s): This is a case of highly unusual Scopulariopsis
gracilis fungal keratitis occurring in the interface between graft and
host following a descemets stripping endothelial keratoplasty in
which a contaminated graft was transplanted. To the best of our
knowledge this is the first time Scopulariopsis gracilis fungal keratitis
is reported and given its incidence following the implantation of a
contaminated endothelial keratoplasty.
Method(s): The infection likely occurred secondary to contamination
of the dextran transport medium in which the human cornea was
transferred from an eye bank. Scopulariopsis gracilis was subsequently
isolated by the reference laboratory.
Results: The slow growth of fungus was only detected on day 3 of
culture. The sample was however released prior to this and implanted
on day 2. Subconjunctival dexamethasone postoperatively may
have encouraged the rapid growth of the fungus. The occular fungal
infiltrates first enlarged despite therapy but later cleared after
changing anti-fungal regime.
Discussion and/or Conclusion(s): Patient had good treatment
response with complete resolution of the fungal keratitis. Diagnosis
was made promptly and treatment initiated early (topical G vorico-
nazole & Amphotericin as well as oral Voriconazole & Itraconazole).
The infected graft was removed promptly with repeat intracameral
injections of anti-fungal agents.
ID: 5153
A prospective surveillance study of airborne Aspergillus fumigatus
in a Tertiary Referral Hospital
Tom Rogers1, Katie Dunne2, Alida Fe Talento3, Niamh Murphy3,
Lisa Fetherstone 3, Catherine O’Reilly3, Brian O’ Connell3. 1Trinity
College & St James’ s Hospital, 2Trinity College Dublin, 3St James’ s
Hospital Dublin
Background: Aspergillus fumigatus is a major fungal pathogen trans-
mitted by the airborne route. Excavation of soil during construction/
renovation is thought to increase the spore burden and risk of noso-
comial acquisition. Air sampling is performed for hospital surveillance,
particularly during major construction to ensure engineering controls
are effective.
Aim(s)/Objective(s): We prospectively collected data over one year on
A. fumigatus counts in order to obtain baseline levels in advance of a
major build.
Method(s): Air sampling was performed twice-monthly at 13 hospital
locations. Indoor locations included an intensive care unit (ICU), a
Stemcell transplant unit, a Cardio-thoracic ICU, and a respiratory ward,
each with different air handling arrangements. A dual head SAS air
sampler was used to sample 1000L of air. Sabouraud dextrose agar was
used to select A. fumigatus colonies, and Roswell Park Memorial
Institute 1640 agar containing itraconazole/voriconazole screened for
triazole resistant A. fumigatus.
Results: Areas with controlled ventilation, and sealed windows,
generally had satisfactory counts. Two wards without controlled
ventilation repeatedly showed high Aspergillus counts which was
addressed by installing portable HEPA filters. The Stem cell transplant
unit consistently had satisfactory results. A. fumigatus CFU counts
outdoors were generally higher than those indoors. Our findings
confirm that adequate environmental control is dependent on having
units/wards with air handling systems that address patients’ risk of
developing nosocomial aspergillosis.
Discussion and/or Conclusion(s): Our results have provided a
valuable baseline in advance of a major construction project and
have allowed for immediate and effective responses in clinical areas
identified with high A. fumigatus counts.
Topic: General
ID: 4449
Exploring needle stick injuries among health-workers in
Mansoura University Hospitals and awareness of medical students
for common types of hepatitis (A, B, and C)
Muhammed Alaa Ahmed Moukhtar Hammad, Lena Osama Shoaib,
Nihal Mustafa Saleh, Mohamed Issam Kabsha,
Mahmoud Magdi Mohamed Elsayed Ali Abdou, Sara Elhusseini,
Sherouk Reda Elsheiwi, Hadeel Mohamed Morad,
Nervana Mohamed Ashraf Khalil, Muhammed Galal Abou Elela,
Hussam Abdulsalam Gad, Nebal Nash’at El-Naqeib,
Maram Adel Abdel-Magied, Mohamed Galal Moawad Mansi,
Rawan Nahel Ahmed, Mariam MohyEldin Shehata,
Amal Ayman Khafagy, Mahmoud Mesbah Ahmed Albiomy,
Mahmoud Salah Al-agouz, Mariam Mohammed Hisham Daba,
Sayed Mohammed Heiba. Mansoura Faculty of Medicine
Background: Hepatitis A, B, and C have been encroaching into Egypt’ s
cities in the recent years.
Aim(s)/Objective(s): Clinical level- Knowing all health-workers who
had needle stick injury in the year 2015 in Mansoura University
Hospitals andwhether they receivedproper vaccination forHepatitis B:
140 health-workers involved after which awareness talk is done to
ensure they are updated on management of possible hepatitis B afflic-
tion. Academical level: Awareness has been done to medical students
and healthcare workers on 3 major hepatitis in Egypt A, B, and C: 903
surveyors.
Method(s): Clinical level: survey on needle stick injuries is given to
health-worker where we ensure confidentiality and then we aware of
hepatitis B. Academic Level: A pre-survey is given to amedical student/
healthworker assessing his/her knowledge of hepatitis A, B, and C.
A general talk targeting certain basic info needed to be known by
students and clinicians is then given. A post survey is then given to
assess the talk.
Results: 53% of 140 health-workers got injured by a needle stick in the
past 12 months. 81% didn’ t file incident report. 90 were not vaccinated
for Hep B and 96 didn’ t receive training in management of needle
stick injury. Out of 903 surveyors for general info on Hep, 40%
answered all questions right in the pre-survey and 90% answered them
right on the post-survey.
Discussion and/or Conclusion(s): More focus is needed to be put on
informing health-workers and medical students on how to prevent
needle stick injury, and they in general need to be aware of the
common types of hepatitis here.
Abstracts of FIS/HIS 2016 – Poster Presentations / Journal of Hospital Infection 94S1 (2016) S24–S134 S94