

ID: 4905
Influence of a probiotic-based sanitizing methods on surface
pathogen persistence and resistance: an effectiveness and safety
study
Elisabetta Caselli
1
, Maria D
’
Accolti
2
, Paola Antonioli
3
, Luca Lanzoni
2
,
Maddalena Coccagna
2
, Maria Teresa Camerada
2
, Sante Mazzacane
2
.
1
University of Ferrara,
2
CIAS, University of Ferrara,
3
S. Anna University
Hospital, Ferrara
Background:
Contamination of hospital surfaces can contribute to
transmission of healthcare-associated infections (HAIs), representing
a global concern. Chemicals-based cleaning shows limitations in
controlling surface bioburden, and can select resistant species.
Recently, a system based on detergents containing probiotics of the
spore-forming
Bacillus
genus (PCHS) was shown to decrease surface
pathogens up to 90% more than conventional disinfectants.
Aim(s)/Objective(s):
Here we wanted to analyse the impact of PCHS
on the drug-resistance features of the hospital surfaces microbiota,
and to assess its safety of use.
Method(s):
Microbial drug-resistance was analysed by a microarray
detecting 84 resistance-genes in the total microbiota and in individual
isolates, and confirmed by conventional antibiograms. Also, a four-
year microbiological surveillance was implemented, evaluating the
presence of
Bacillus
strains in all the clinical samples of the patients
admitted to PCHS-treated hospitals.
Results:
PCHS use induced a drop (up to
−
99%) of resistant pathogen
strains, rather than selecting them. Notably, detergent-
Bacillus
strains
remained genetically unmodified even after years of continuous
contact with surface pathogens, and no infections attributable to
Bacillus
were observed in 32,139 analysed samples from seven
hospitals.
Discussion and/or Conclusion(s):
Results show that PCHS application
can effectively reduce the number of pathogens and their drug
resistance. Furthermore, the genetic stability of PCHS-
Bacillus
trains,
together with the absence of HAI attributable to probiotic
Bacilli
suggest that they do not have the ability to cause infections, even in the
subjects at higher risk for adverse events, such as hospitalized
patients, and support the safety of environmental use of probiotics
for sanitation purposes.
Topic: Environment
ID: 4420
Transfer frequency of dry surface biofilm in health-care
environment
–
the role of health care worker
’
s hand
Durdana Chowdhury, Shamaila Tahir, Mark Legge, Honghua Hu,
Khalid Aljohani, Anand Deva, Karen Vickery.
Macquarie University
Background:
Hospital environmental surfaces become contaminated
by pathogens shed by infected patients and can survive for extended
periods when incorporated into dry surface biofilms (DSB). Within
biofilms, bacteria are protected from desiccation and increased
tolerance to removal by cleaning agents and disinfectants. We
hypothesise that pathogens in DSB play a significant role in the
endemic transmission of healthcare-associated infections.
Aim(s)/Objective(s):
This study aims to determine if DSB can be
transmitted from hospital surface to healthcare worker
’
s hands.
Method(s):
DSB of
Staphylococcus aureus
was grown in vitro on
polycarbonate and glass coupons in CDC bioreactor over a period of 12
days with periodic nutrition interspersed with long periods of
dehydration. Each coupon had 10^
7
bacterial cells. Transmission was
tested using gloved and ungloved hands via various materials (plastic
and glass coupon and cotton sheet) before and after treatment with 5%
neutral detergents.
Results:
Transmission of organisms was 10 times higher with bare
hands than gloved hands. Both nitrile gloves and surgical gloves
transmitted approximately 6 times the bacteria than latex gloves.
Following hand contamination with DSB, transmission to 20
subsequent surfaces was highly possible. Cotton bed sheet also
showed higher rate of transmissibility. It is astonishing that, 7.3%
organism can pass through cotton sheet to contaminate skin of hands.
Surprisingly, DSB treated with 5% neutral detergent increased the
transmission rate of DSB bacteria tenfold.
Discussion and/or Conclusion(s):
DSB is highly transmissible. It is
higher with bare hand and can be transmitted to the patient from bed
mattress, through the bed sheet.
ID: 4503
Non-slip socks: A potential reservoir for transmitting multidrug
resistant organisms in hospitals?
Nikunj (Nik) Mahida, Tim Boswell.
Nottingham University Hospitals
Background:
Falls and fall-related injuries are common within acute
healthcare settings. In response to patients admitted with unsuitable
or inappropriate footwear, a number of hospitals have introduced non-
slip socks to reduce falls.
Aim(s)/Objective(s):
This study evaluates whether non-slip socks can
be become contaminated with multidrug resistant organisms found
on the floor.
Method(s):
54 pairs of used socks and 35 environmental floor samples
were obtained from 7 wards in a tertiary referral hospital. Socks were
anonymously collected frompatients by offering a new pair of non-slip
socks in exchange for the in-use pair. On each ward where socks were
collected, five floor areas (2 ward corridors, 3 toilets) were sampled
using a Polywipe
™
sponge.
Results:
Vancomycin resistant enterococci (VRE) were detected from
46 socks (85%) and Meticillin-resistant Staphylococcus aureus (MRSA)
from 5 (9%). Environmental sampling cultured VRE from 24 floor
samples (69%) and MRSA from 6 floor samples (17%). Clostridium
difficile was not detected from any sample.
Discussion and/or Conclusion(s):
The study demonstrates that non-
slip socks can become contaminated with multidrug resistant
pathogens. Patients not only use them to walk to various parts of the
hospital during the inpatient journey but also wear them in bed. It
suggests a potential route for cross-transmission, which thus far has
not been considered.
ID: 4550
Comparison of environmental sampling methods for the
investigation of an outbreak of Group A Streptococcus
Amelia Joseph
1
, Vivienne Weston
2
.
1
Nottingham University Hospitals/
Health Education East Midlands,
2
Nottingham University Hospitals
Background:
There is no guidance on the optimal method of sampling
the non-healthcare environment for Group A Streptococcus (GAS)
during an outbreak.
Aim(s)/Objective(s):
To compare methods of sampling soft furnish-
ings in a residential home setting during an outbreak of GAS.
Method(s):
Three cases of GAS infection (emm type st1.0) and eight
asymptomatic cases were identified over a four month period in a
residential home setting. During the outbreak we sampled soft
furnishings (carpets, curtains and soft furniture) using three
methods: standard swabs onto blood agar; Polywipe
®
sponges into
brain heart infusion broth with subculture to streptococcal selective
agar; and blood agar sweep-plates. Isolation of GAS was compared at
24 and 48 hours.
Results:
All standard swab samples were negative. GAS was isolated
from the patients
’
carpets by sweep-plate method in three, and by
Polywipe
®
sponge method in two, of the four samples. Curtains in one
patient
’
s room and in the communal living room were positive by
sweep-plate method, but negative by Polywipe
®
sponge method. A
footstool in the communal living room was positive by both sweep-
plate and Polywipe
®
sponge methods. There were no additional
positives at 48 hours compared to 24 hours of incubation. All
environmental isolates were emm type st1.0.
Discussion and/or Conclusion(s):
The sweep-plate method is a
rapid and effective way of sampling soft furnishings for GAS.
Abstracts of FIS/HIS 2016
–
Oral Presentations / Journal of Hospital Infection 94S1 (2016) S11
–
S21
S18